Detection methods

 Endogenous methods

Accurate detection of GM events by PCR requires the use of appropriate controls.

One such control is a taxon-specific assay that amplifies a specific sequence of known copy number and
demonstrates no allelic variation in the host plant genome, also referred to as an endogenous
method.

CropLife International member companies (BASF, Bayer, Corteva, and Syngenta) have
chosen to make a number of validated endogenous methods publicly available, to support
global harmonization of detection methods, to reduce development costs for academics and
small/medium enterprises and to ensure that the CropLife International Detection Methods
Database remains a comprehensive and trusted resource for detection methods.

Crop 

Taxon /
Endogenous method Assay
 

Base pairs 

Companies

Canola/Oilseed Rape 

CruA 

101 

  1. BASF
  2. Corteva Agriscience 

Canola/Oilseed Rape 

FatA(A) 

126 

  1. Corteva Agriscience 

Corn/Maize 

ADH1 

135 

  1. BASF
  2. Syngenta

Corn/Maize 

HMG 

79 

  1. Bayer CropScience
  2. Corteva Agriscience

Cotton 

AdhC 

73 

  1. BASF
  2. Corteva Agriscience  

Cotton 

SAH7 

115 (A Genome) 

123 (D Genome) 

  1. Corteva Agriscience
  2. Syngenta  

Soybean 

LE1 

74 

  1. Bayer CropScience
  2. Corteva Agriscience 
  3. Syngenta  

Soybean 

LE1 

102 

  1. BASF 

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